Recombinant Cyclin D3 Monoclonal Antibody

Specifications

Catalog number

AN301504L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

Abbreviation

Cyclin D3

UniProt

P30281

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human Cyclin D3 fragment

Target

G1 S specific cyclin D; G1/S specific cyclin D; G1/S-specific cyclin-D; CyclinD; cyclin D; CCND3; cyclin D3; CCND 3; CyclinD3; D3 type cyclin; G1 S specific cyclin D3; G1/S specific cyclin D3; G1/S-specific cyclin-D3

Clonality

Monoclonal, Recombinant

Clone

A203

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Cancer

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:50-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

33 kDa

Observed Molecular Weight

33 kDa

Isotype

IgG, κ