Recombinant eIF3B Monoclonal Antibody

Specifications

Catalog number

AN301513L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. UniRule annotation4 Publications (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.

Abbreviation

EIF3B

UniProt

P55884

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Recombinant human eIF3B1 fragment

Target

PRT; EIF3S; EIF3B; EIF3-ETA; EIF3-P110; EIF3-P116; EIF3S9; PRT1

Clonality

Monoclonal, Recombinant

Clone

A212

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:1000; IHC 1:50-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

92 kDa

Observed Molecular Weight

116 kDa

Isotype

IgG, κ