Recombinant SATB1 Monoclonal Antibody

Specifications

Catalog number

AN301652L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g., PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation.

Abbreviation

SATB1

UniProt

Q01826

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human SATB1 fragment

Target

SATB; SATB1

Clonality

Monoclonal, Recombinant

Clone

A355

Conjugation

Unconjugated

Applications

WB; IHC; FCM

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:200-1:1000; FCM 1:50-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

86 kDa

Observed Molecular Weight

100 kDa

Isotype

IgG, κ