Recombinant Smac/Diablo Monoclonal Antibody

Specifications

Catalog number

AN301658L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP) . Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

Abbreviation

Smac/Diablo

UniProt

Q9NR28

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Recombinant human Smac/Diablo fragment

Target

DFNA; DIABLO; DFNA64; SMAC;0610041G12Rik; DBLOH; DBOH; Diablo homolog; Diablo homolog (Drosophila) ; Diablo homolog mitochondrial; Diablo IAP binding mitochondrial protein; Diablo like protein; DIABLO S; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second mitochondria derived activator of caspase; Second mitochondria-derived activator of caspase; SMAC 3; Smac protein; SMAC3

Clonality

Monoclonal, Recombinant

Clone

A361

Conjugation

Unconjugated

Applications

WB; IHC; IF; FCM; IP

Field of Research

Cell Biology; Cancer; Metabolism

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:50-1:100; IF 1:500-1:1000; FCM 1:50-1:100; IP 1:25-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

27 kDa

Observed Molecular Weight

21 kDa

Isotype

IgG, κ