Recombinant TPX2 Monoclonal Antibody

Specifications

Catalog number

AN301679L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules.Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha .At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.

Abbreviation

TPX2

UniProt

Q9ULW0

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Recombinant human TPX2 fragment

Target

FLS; HCA; TPX; HCTP; REPP; GD:C20orf; DIL; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1; TPX2; Chromosome 20 Open Reading Frame 1; Differentially expressed in cancerous and noncancerous lung cells 2; Differentially expressed in cancerous and non-cancerous lung cells 2; Differentially expressed in lung cells; Differentially expressed in lung cells 2; DIL 2; HCA 519; HCTP 4; Hepatocellular carcinoma associated antigen 519; Hepatocellular carcinoma-associated antigen 519; Preferentially expressed in colorectal cancer; Protein fls353; REPP 86; Restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; Targeting protein for Xklp2; TPX 2; TPX2 microtubule associated homolog; TPX2 microtubule associated protein homolog; TPX2 microtubule nucleation factor

Clonality

Monoclonal, Recombinant

Clone

A382

Conjugation

Unconjugated

Applications

IHC; IF; FCM; WB

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Cancer

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:1000; IHC 1:50-1:200; IF 1:50; FCM 1:50-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

86 kDa

Observed Molecular Weight

100 kDa

Isotype

IgG, κ