Recombinant ILF3 Monoclonal Antibody

Specifications

Catalog number

AN301758L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin enhancer-binding factor 3 (ILF3) is a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa) . Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response.

Abbreviation

ILF3

UniProt

Q12906

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Recombinant human ILF3 fragment

Target

ILF; MMP; Mpp; DRBP; NF-AT; MPHOSPH; TCP; ILF3; CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; NF-AT-90; NF110; NF110b; NF90; NF90a; NF90b; NFAR; NFAR-1; NFAR2; TCP110; TCP80; Double stranded RNA binding protein 76; Double-stranded RNA-binding protein 76; Interleukin enhancer binding factor 3; Interleukin enhancer-binding factor 3; M phase phosphoprotein 4; M-phase phosphoprotein 4; NF AT 90; Nuclear factor associated with dsRNA; Nuclear factor of activated T cells 90 kDa; Nuclear factor of activated T-cells 90 kDa; Translational control protein 80

Clonality

Monoclonal, Recombinant

Clone

A466

Conjugation

Unconjugated

Applications

WB; IHC; IF; FCM

Field of Research

Signal Transduction; Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:2000-1:10000; IHC 1:100-1:500; IF 1:50; FCM 1:50-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

95 kDa

Observed Molecular Weight

90,110 kDa

Isotype

IgG, κ