Recombinant IKK gamma Monoclonal Antibody

Specifications

Catalog number

AN301804L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

IKK gamma (IKKγ) is a subunit of a high molecular weight IκB kinase (IKK) complex, which is involved in NF-κB activation. The catalysis of IκB kinase (IKK) complex is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation. Activation of the NF-κB pathway by the T-cell lymphotrophic virus Tax protein or by TNF-α treatment leads to IKKβ-dependent phosphorylation of human IKKγ, primarily at Ser376. In mice, mutation of the orthologous residue (Ser369) to alanine leads to enhanced IKKγ-mediated stimulation of IKKβ kinase activity.

Abbreviation

IKK gamma

UniProt

Q9Y6K9

Host

Rabbit

Reactivity

Human; Mouse

Immunogen

Recombinant human IKK gamma fragment

Target

IMD; IPD; AMCBX; IKKAP; ZC2HC; FIP; IKBKG; AMCBX1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; ZC2HC9; NF-kappa-B essential modulator; FIP 3; gamma; I kappa B kinase gamma; I-kappa-B kinase subunit gamma; IkB kinase associated protein 1; IkB kinase gamma subunit; IkB kinase subunit gamma; IkB kinase-associated protein 1; Incontinentia pigmenti; Inhibitor of kappa light polypeptide gene enhancer in B cells; Inhibitor of nuclear factor kappa B kinase subunit gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; kinase gamma; kinase of; NF kappa B essential modifier; NF kappa B essential modulator; NF-kappa-B essential modifier

Clonality

Monoclonal, Recombinant

Clone

A516

Conjugation

Unconjugated

Applications

WB; IF; FCM; IP

Field of Research

Cell Biology; Signal Transduction; Epigenetics and Nuclear Signaling; Cardiovascular; Immunology; Cancer

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IF 1:50; FCM 1:50-1:100; IP 1:25-1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

48 kDa

Observed Molecular Weight

48 kDa

Isotype

IgG, κ