Recombinant HMGB2 Monoclonal Antibody

Specifications

Catalog number

AN301821L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

HMGB2 belongs to a family of highly conserved proteins that contain HMG box domains. HMGB2 is widely expressed during embryonic development, but it is restricted to lymphoid organs and testis in adult animals. HMGB2 facilitate the binding of Hox proteins, Oct proteins, p53, Rel proteins, and steroid hormone receptor proteins to their target gene promoters. Furthermore, HMGB2 interacts with RAG1 to facilitate RAG complex binding to the recombinant signal sequence (RSS) and stimulate DNA-bending and subsequent VDJ cleavage at antigen receptor genes. In addition to their functions in the nucleus, HMGB proteins play a significant role in extracellular signaling associated with inflammation. HMGB2 is secreted by myeloid cells and promotes proliferation and migration of endothelial cells by binding to the receptor for advanced glycation end products (RAGE) .

Abbreviation

HMGB2

UniProt

P26583

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Recombinant human HMGB2 fragment

Target

HMGB; High mobility group box; High mobility group (nonhistone chromosomal) protein; HMG B; HMG; HMGB2; HMG2; C80539; High mobility group (nonhistone chromosomal) protein 2; High mobility group box 2; High mobility group protein 2; High mobility group protein B2; HMG 2; HMG B2; HMG-2

Clonality

Monoclonal, Recombinant

Clone

A533

Conjugation

Unconjugated

Applications

WB; IHC; IF; IP

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:1000; IHC 1:50-1:100; IF 1:50; IP 1:25-1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

24 kDa

Observed Molecular Weight

24 kDa

Isotype

IgG, κ