Recombinant ATF1 Monoclonal Antibody

Specifications

Catalog number

AN301850L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATF-1 is a bZIP transcription factor that is a member of the cyclic AMP (cAMP) -response element-binding protein (CREB) family. Like other members of the CREB family, ATF-1 mediates signals from numerous physiological stimuli and activates target genes through cAMP-response elements, resulting in the regulation of a broad array of cellular responses. ATF-1 can be phosphorylated at Ser63 by protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), and cyclin-dependent kinase 3 (CDK3), which recruits CBP/p300 and enhances transcriptional activity. ATF-1 expression levels have been found to be elevated in lymphomas and metastatic melanoma cells, where it contributes to tumor growth.

Abbreviation

ATF1

UniProt

P81269

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Recombinant human ATF1 fragment

Target

ATF; Atf1

Clonality

Monoclonal, Recombinant

Clone

A562

Conjugation

Unconjugated

Applications

WB; IHC; IF; FCM

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:1000; IHC 1:100-1:1000; IF 1:50; FCM 1:50-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

29 kDa

Observed Molecular Weight

35 kDa

Isotype

IgG, κ