Recombinant SHIP-1 Monoclonal Antibody

Specifications

Catalog number

AN301886L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate. SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus. Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif, followed by tyrosine phosphorylation on the NPXY motif. The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1. Its effect on calcium flux, cell survival, growth, cell cycle arrest, and apoptosis is mediated through the PI3K and Akt pathways. Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function.

Abbreviation

SHIP-1

UniProt

Q92835

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human SHIP-1 fragment

Target

SIP; INPP5D; SHIP; SHIP-1; SHIP1; SIP-145; hp51CN; p150Ship

Clonality

Monoclonal, Recombinant

Clone

A602

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Immunology; Signal Transduction

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:1000; IHC 1:200-1:1000; IF 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

133 kDa

Observed Molecular Weight

133 kDa

Isotype

IgG, κ