Recombinant ROCK2+ROCK1 Monoclonal Antibody

Specifications

Catalog number

AN301910L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity. ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence resulting in loss of its C-terminal inhibitory domain. ROCK2 is directly cleaved by granzyme B (grB) . Cleavage activates ROCK2 and leads to phosphorylation of myosin light chain (MLC) and inhibition of myosin phosphatase. This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction.

Abbreviation

ROCK2+ROCK1

UniProt

O75116; Q13464

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Recombinant human ROCK2+ROCK1 fragment

Target

Rho-associated protein kinase 2; ROCK-II; p160ROCK; ROCK-I

Clonality

Monoclonal, Recombinant

Clone

A626

Conjugation

Unconjugated

Applications

WB; IHC; IF

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:1000; IHC 1:50-1:100; IF 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

161, 158 kDa

Observed Molecular Weight

161 kDa

Isotype

IgG, κ