Recombinant eIF3G Monoclonal Antibody

Specifications

Catalog number

AN301973L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

EIF3 is a large translation initiation complex with 10 to 13 different subunits. eIF3A, eIF3B, eIF3C, eIF3E, eIF3F, and eIF3H are the core subunits critical for the function of this complex. eIF3 physically interacts with eIF4G, which may be responsible for the association of the 40S ribosomal subunit with mRNA. eIF3 also stabilizes the binding of Met-tRNAf.eIF2.GTP to the 40S ribosomal subunit and helps keep the integrity of the resulting complex upon addition of the 60S ribosomal subunit. Studies have shown that mTOR interacts with eIF3 directly. When cells are stimulated by hormones or mitogenic signals, mTOR binds to the eIF3 complex and phosphorylates S6K1. This process results in the dissociation of S6K1 from eIF3 and S6K1 activation. The activated S6K1 then phosphorylates its downstream targets including ribosomal protein S6 and eIF4B, resulting in stimulation of translation. Further findings demonstrated that activated mTOR signaling induces the association of eIF3 with eIF4G upon stimulation with insulin.

Abbreviation

EIF3G

UniProt

O75821

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Peptide. This information is proprietary to PTMab.

Target

EIF3S; EIF3G; EIF3-P42; EIF3S4; eIF3-delta; eIF3-p44

Clonality

Monoclonal, Recombinant

Clone

A689

Conjugation

Unconjugated

Applications

WB; IHC; IF; FCM

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:1000-1:2000; IHC 1:50-1:100; IF 1:50-1:100; FCM 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

36 kDa

Observed Molecular Weight

40 kDa

Isotype

IgG, κ