Recombinant Ryanodine Receptor Monoclonal Antibody

Specifications

Catalog number

AN301983L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ryanodine receptors (RyRs) are large (>500 kDa), intracellular calcium channels found in the sarcoplasmic/endoplasmic reticulum membrane and are responsible for the release of Ca2+ from intracellular stores in excitable cells, such as muscle and neurons. RyRs exist as three mammalian isoforms (RyR1-3), all of which form homotetramers regulated by phosphorylation and/or direct or indirect interaction with a variety of proteins (L-type calcium channels, PKA, FKBP12/12.6, CaMKII, calmodulin, calsequestrin, junctin, and triadin) and ions (Mg2+ and Ca2+) . Regulation of the RyR channel by protein modulators occurs within the large cytoplasmic domain, whereas the carboxy terminal portion of the protein forms the ion-binding and conducting pore. RyR1 and RyR2 are predominantly expressed in skeletal and cardiac muscle, respectively, where they localize exclusively to the sarcoplasmic reticulum (SR) and facilitate calcium-mediated communication between transverse-tubules and sarcoplasmic reticulum. Contraction of skeletal muscle is triggered by release of calcium ions from the SR following depolarization of T-tubules. Research studies have shown that defects in RyR1 are the cause of malignant hyperthermia susceptibility type 1 (MHS1), central core disease of muscle (CCD), multiminicore disease with external ophthalmoplegia, and congenital myopathy with fiber-type disproportion (CFTD), each of which is manifested by defects in muscle function, metabolism, and development.

Abbreviation

Ryanodine Receptor

UniProt

P21817

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Peptide. This information is proprietary to PTMab.

Target

PPP1R; CCO; KDS; MHS; RYR; MHS1; RYDR; SKRR; RYR-1; PPP1R137; RYR1

Clonality

Monoclonal, Recombinant

Clone

A703

Conjugation

Unconjugated

Applications

IHC

Field of Research

Neuroscience; Cardiovascular; Cancer; Metabolism

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

IHC 1:500-1:1000

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Isotype

IgG, κ