Recombinant APAF1 Monoclonal Antibody

Specifications

Catalog number

AN301989L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis. Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3. The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat. Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli.

Abbreviation

APAF1

UniProt

O14727

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Peptide. This information is proprietary to PTMab.

Target

KIAA; Apoptotic protease-activating factor; Apoptotic peptidase activating factor; CED; APAF1; APAF-1; CED4; APAF; APAF 1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor; Apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED 4; KIAA0413

Clonality

Monoclonal, Recombinant

Clone

A709

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Cell Biology; Cancer; Metabolism

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:1000-1:3000; IHC 1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

142 kDa

Observed Molecular Weight

140 kDa

Isotype

IgG, κ