Recombinant Nucleolin Monoclonal Antibody

Specifications

Catalog number

AN302000L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nucleolin is a multi-functional protein that is one of the major components of the nucleoli. Nucleolin plays an essential role in various steps of ribosome biogenesis including rRNA synthesis, processing of pre-rRNA, pre-ribosomal RNA assembly, and transport of ribosomal proteins out of the nucleus.Down regulation of nucleolin leads to increased expression of p53, defects in genome duplication, and a delay at prometaphase during mitosis leading to cell cycle arrest. In addition, nucleolin has been found in a complex with Rad51 and may participate in DNA repair by homologous recombination. Nucleolin binds to the catalytic subunit of the human telomerase reverse transcriptase, hTERT, and is thought to be involved in telomere maintenance. Nucleolin also possesses histone chaperone activity and is able to enhance the chromatin remodeling efficiency of SWItch/Sucrose Non Fermentable (SWI/SNF) and ATP-dependent chromatin-assembly factor (ACF), remove histone H2A-H2B dimers from nucleosomes, and facilitate the passage of RNA polymerase through chromatin.

Abbreviation

Nucleolin

UniProt

P19338

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Peptide. This information is proprietary to PTMab.

Target

Nsr; NCL; C23; Nsr1; nucleolin

Clonality

Monoclonal, Recombinant

Clone

A720

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Tags & Cell Markers; Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:100000-1:1000000; IHC 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

77 kDa

Observed Molecular Weight

110 kDa

Isotype

IgG, κ