Recombinant CIRBP Monoclonal Antibody

Specifications

Catalog number

AN302011L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cold-induced RNA-binding protein (CIRBP) is a 172-residue, multifunctional sensor protein that was first isolated as a protein induced in mouse fibroblasts cultured at 32ºC. Conversely, CIRBP expression decreases in cells or tissues subjected to increased temperature. The CIRBP protein is composed of an amino-terminal RNA-binding domain and a carboxyl-terminal, glycine-rich domain. Stressful stimuli, such as hypoxia, heat shock, osmotic shock, or oxidative conditions, lead to translocation of CIRBP from the nucleus to cytoplasmic stress granules through a mechanism involving CIRBP methylation-dependent nuclear export. CIRBP plays a role in regulating apoptosis and preserving the stemness of neural stem cells at moderately low temperatures. Research studies demonstrate that CIRBP contributes to the regulation of circadian rhythm through post-translational modulation of CLOCK expression.

Abbreviation

CIRBP

UniProt

Q14011

Host

Rabbit

Reactivity

Human

Immunogen

Peptide. This information is proprietary to PTMab.

Target

CIRBP; CIRP; A18HNRNP

Clonality

Monoclonal, Recombinant

Clone

A731

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Epigenetics and Nuclear Signaling; Cell Biology

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:1000; IHC 1:500-1:1000; IF 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

19 kDa

Observed Molecular Weight

16 kDa

Isotype

IgG, κ