Recombinant SHIP-2 Monoclonal Antibody

Specifications

Catalog number

AN302029L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate. SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus. Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif, followed by tyrosine phosphorylation on the NPXY motif. The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1. Its effect on calcium flux, cell survival, growth, cell cycle arrest, and apoptosis is mediated through the PI3K and Akt pathways. Tyr1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function.SHIP2, a homolog of SHIP1, is highly expressed in heart, skeletal muscle and placenta.SHIP2 negatively regulates insulin signaling and polymorphisms in SHIP2 have been linked to hyperglycemia. Recent studies also suggest SHIP2 as a therapeutic target for the treatment of both obesity and type 2 diabetes. Tyr1135 is phosphorylated in human cancer cells.

Abbreviation

SHIP-2

UniProt

O15357

Host

Rabbit

Reactivity

Human

Immunogen

Peptide. This information is proprietary to PTMab.

Target

SHIP; INPPL; INPPL1; OPSMD; SHIP2;5 trisphosphate 5 phosphatase 2;51C protein;5-trisphosphate 5-phosphatase 2; EC 3.1.3.n1; inositol polyphosphate phosphatase like 1; Inositol polyphosphate phosphatase like protein 1; Inositol polyphosphate phosphatase-like protein 1; INPPL-1; Phosphatidylinositol 3; Protein 51C; SH2 domain containing inositol 5' phosphatase 2; SH2 domain-containing inositol 5''-phosphatase 2; SH2 domain-containing inositol phosphatase 2; SHIP-2

Clonality

Monoclonal, Recombinant

Clone

A749

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Cardiovascular; Signal Transduction; Immunology; Neuroscience

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:1000; IHC 1:100; IF 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

139 kDa

Observed Molecular Weight

140 kDa

Isotype

IgG, κ