Recombinant SMARCC2/BAF170 Monoclonal Antibody

Specifications

Catalog number

AN302065L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) .Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by alteringDNA-histone contacts within a nucleosome in an ATP-dependent manner. BAF170 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by Brg1 in vitro. While BAF170 has been shown to be part of the SWI/SNF complexin non-pluripotent cells, it is absent in pluripotent embryonic stem (ES) cells. Research studies have shown that expression of BAF170 is upregulated in neurons/neuronal progenitors upon differentiation of mouse ES cells with retinoic acid, and exogenous expression of BAF170leads to loss of stem cell pluripotency and self renewal.

Abbreviation

SMARCC2/BAF170

UniProt

Q8TAQ2

Host

Rabbit

Reactivity

Human; Mouse

Immunogen

Peptide. This information is proprietary to PTMab.

Target

Rsc; BAF; CRACC; SMARCC; SMARCC2; BAF170; CRACC2; Rsc8

Clonality

Monoclonal, Recombinant

Clone

A785

Conjugation

Unconjugated

Applications

WB; IHC; FCM; IP

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:1000-1:2000; IHC 1:500-1:1000; FCM 1:100; IP 1:50-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

133 kDa

Observed Molecular Weight

170 kDa

Isotype

IgG, κ