Recombinant Phospho-S6 Ribosomal Protein (Ser235, Ser236) Monoclonal Antibody

Specifications

Catalog number

AN302076L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ribosomal Protein S6 (RPS6), a highly conserved component of the eukaryotic 40S small ribosomal subunit, functions as a critical regulatory nexus that translates extracellular and intracellular signals into the control of cell growth and proliferation. This activity is tightly governed by the PI3K/Akt/mTOR signaling cascade, a master regulatory pathway that integrates inputs from growth factors and nutrient availability. In response to mitogenic or anabolic stimuli that activate this pathway, the downstream effector kinase p70 S6K1 mediates the hierarchical phosphorylation of RPS6 on a cluster of serine residues located in its C-terminus, with the phosphorylation of the adjacent serines 235 and 236 representing a canonical hallmark of mTORC1 activation. This specific post-translational modification is mechanistically linked to the primary regulatory function of RPS6, which is to facilitate the selective and efficient translation of a specific cohort of messenger RNAs characterized by a 5' terminal oligo-pyrimidine tract (5' TOP) .

Abbreviation

Phospho-S6 Ribosomal Protein (Ser235, Ser236)

UniProt

P62753

Host

Rabbit

Reactivity

Human; Mouse

Immunogen

Phosphorylated human S6 ribosomal protein (Ser235/236) peptide

Target

RPS; OK/SW-cl; RPS6; S6; OK/SW-cl.2

Clonality

Monoclonal, Recombinant

Clone

A796

Conjugation

Unconjugated

Applications

WB; IHC; IF

Field of Research

Epigenetics and Nuclear Signaling

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:500-1:1000; IHC 1:200-1:1000; IF 1:50

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

29 kDa

Observed Molecular Weight

32 kDa

Isotype

IgG, κ