Recombinant Phospho-PAK1 (Thr212) Monoclonal Antibody

Specifications

Catalog number

AN302100L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth. Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK. Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1. Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1, and Ser192 and Ser197 of PAK2. Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation. Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity. Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck. PAK4, PAK5/7, and PAK6 have lower sequence similarity with PAK1-3 in the amino terminal regulatory region. Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4. PAK family members are widely expressed, and often overexpressed in human cancer.

Abbreviation

Phospho-PAK1 (Thr212)

UniProt

Q13153

Host

Rabbit

Reactivity

Human; Mouse

Immunogen

Peptide. This information is proprietary to PTMab

Target

PAK; PAK1; PAKalpha; ADRB2; Alpha PAK; Alpha-PAK; MGC130000; MGC130001; p21 activated kinase 1; p21 protein (Cdc42/Rac) activated kinase 1; p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) ; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog; p21-activated kinase 1; p65 PAK; p65-PAK; P68-PAK; PAK alpha; PAK-1; Paka; Protein kinase MUK2; Rac/p21-activated kinase; Serine/threonine-protein kinase PAK 1; STE20 homolog yeast; yeast)

Clonality

Monoclonal, Recombinant

Clone

A824

Conjugation

Unconjugated

Applications

WB

Field of Research

Cell Biology; Signal Transduction; Cancer; Microbiology; Neuroscience

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:1000-1:5000

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

61 kDa

Observed Molecular Weight

62 kDa

Isotype

IgG, κ