Recombinant Histone H3 (Mono Methyl Lys79) Monoclonal Antibody

Specifications

Catalog number

AN302113L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

The ε-amino lysine acetylation of proteins is an important reversible modification controlling protein activity. The amino-terminal tails of core histones undergo lysine methylation in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation in histones modulated by specific histone methyltransferases (HMTs) and histone demethylases (HDMs) is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone lysine methylation have become promising targets for anti-cancer drugs.

Abbreviation

Histone H3 (Mono Methyl Lys79)

UniProt

P68431

Host

Rabbit

Reactivity

Human; Rat; Mouse

Immunogen

Monomethylated human histone H3 (Lys79) peptide

Target

Histone H; H3C; H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; H3C1; Histone H3.1; Histone H3/a; Histone H3/b; H3FL; HIST1H3B; H3FC; HIST1H3C; H3FB; HIST1H3D; H3FD; HIST1H3E; H3FI; HIST1H3F; H3FH; HIST1H3G; H3FK; HIST1H3H; H3FF; HIST1H3I; H3FJ; HIST1H3J; H3/A; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; Histone H3.1; Histone H3/a; Histone H3/b; H3 histone family; H31; H3a; histone 1; Histone cluster 1; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; member A

Clonality

Monoclonal, Recombinant

Clone

A837

Conjugation

Unconjugated

Applications

WB; IHC

Field of Research

Epigenetics and Nuclear Signaling; Isotype; Loading Controls

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:2000; IHC 1:50-1:100

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

15 kDa

Observed Molecular Weight

15 kDa

Isotype

IgG, κ