Recombinant Histone H3 (Mono Methyl Arg2) Monoclonal Antibody

Specifications

Catalog number

AN302117L-01

Size / count

50 µL

Dry ice

No

Hazardous

No

Discontinued

No

Background

Histone post-translational modifications (PTMs) are key mechanisms of epigenetics that modulate chromatin structures, termed as “histone code”. The PTMs on histone including acetylation, methylation, phosphorylation and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability, gene transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core histones. Methylation of histones can either increase or decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, Trimethylation on Lys, mono-di-symmetric/asymmetric methylation on Arg) . Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. histone methyltransferases (HMTs) and histone demethylases (HDMs) are major regulating factors.

Abbreviation

Histone H3 (Mono Methyl Arg2)

UniProt

P68431

Host

Rabbit

Reactivity

Human; Rat; Mouse; Monkey; Pig; Arabidopsis thaliana; Phaeodactylum tricornutum

Immunogen

Monomethylated human histone H3 (Arg2) peptide

Target

Histone H; H3C; H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; H3C1; Histone H3.1; Histone H3/a; Histone H3/b; H3FL; HIST1H3B; H3FC; HIST1H3C; H3FB; HIST1H3D; H3FD; HIST1H3E; H3FI; HIST1H3F; H3FH; HIST1H3G; H3FK; HIST1H3H; H3FF; HIST1H3I; H3FJ; HIST1H3J; H3/A; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; Histone H3.1; Histone H3/a; Histone H3/b; H3 histone family; H31; H3a; histone 1; Histone cluster 1; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; member A

Clonality

Monoclonal, Recombinant

Clone

A841

Conjugation

Unconjugated

Applications

WB; IF

Field of Research

Epigenetics and Nuclear Signaling; Isotype; Loading Controls

Purification

Protein A purified

Concentration

1 mg/mL

Dilution

WB 1:1000-1:2000; IF 1:100-1:400

Buffer

PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Shipping Conditions

Ice bag

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

15 kDa

Observed Molecular Weight

15 kDa

Isotype

IgG, κ