NEB Stable Competent E. coli (High Efficiency)

Catalog number C3040H

Price for C3040H

Size / count 20 Assays

About this product

NEB Stable Competent E. coli (High Efficiency), C3040H, are chemically competent E. coli cells designed for high-efficiency transformation and cloning of unstable DNA inserts, repetitive sequences and plasmids. The strain is particularly suitable for maintaining difficult constructs and for isolating and propagating retroviral and lentiviral clones. With a transformation efficiency of approximately 1-3 × 10⁹ cfu/µg pUC19 DNA, NEB Stable competent cells combine high transformation performance with reduced recombination of cloned DNA. Key Features <div><ul><li>High-efficiency chemically competent E. coli.</li><li>Ideal for unstable inserts and repetitive DNA sequences.</li><li>Suitable for retroviral and lentiviral plasmid cloning.</li><li>recA1 mutation helps reduce unwanted DNA recombination.</li><li>endA1 mutation supports high-quality plasmid DNA preparation.</li><li>Compatible with NEBuilder HiFi DNA Assembly, Gibson Assembly and ligation reactions.</li><li>Supplied with optimized NEB 10-beta/Stable Outgrowth Medium.</li><li>T1 phage resistant and free of animal products.</li></ul></div>

Specifications

Catalog number: C3040H
Size / count: 20 Assays
Dry ice: No
Hazardous: No
Discontinued: No
Applications: NEB Stable Competent E. coli C3040H is recommended for high-efficiency transformation, plasmid cloning, cloning unstable DNA, repeat-element cloning, lentiviral vector cloning, retroviral vector cloning and DNA assembly workflows. It is especially useful when conventional cloning strains have difficulty maintaining constructs containing direct repeats, inverted repeats or other recombination-prone DNA sequences.
FAQ: What is C3040H? => C3040H is the catalog number for the 20 × 50 µL format of NEB Stable Competent E. coli (High Efficiency), a chemically competent cloning strain optimized for unstable and repetitive DNA constructs. What is NEB Stable Competent E. coli used for? => It is used for high-efficiency transformation and for cloning unstable inserts, repetitive DNA sequences, retroviral constructs and lentiviral constructs. What is the transformation efficiency of C3040H? => NEB Stable Competent E. coli has a specified transformation efficiency of approximately 1–3 × 10⁹ cfu/µg. Can C3040H be used for Gibson Assembly? => Yes. NEB lists the strain as compatible with Gibson Assembly, NEBuilder HiFi DNA Assembly and standard ligation reactions. Why are NEB Stable cells useful for unstable plasmids? => The strain contains recA1, which reduces recombination of cloned DNA, helping maintain repetitive and otherwise unstable plasmid constructs.

Frequently Asked Questions

What is the catalog number for NEB Stable Competent E. coli (High Efficiency)?

The catalog number for NEB Stable Competent E. coli (High Efficiency) is C3040H.

What sizes are available for NEB Stable Competent E. coli (High Efficiency)?

NEB Stable Competent E. coli (High Efficiency) is available in the following size(s): 20 Assays.

Is NEB Stable Competent E. coli (High Efficiency) in stock?

NEB Stable Competent E. coli (High Efficiency) is currently made to order / not held in immediate stock. Contact us for lead time.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.