IRF9 Recombinant Rabbit Monoclonal Antibody [PSH08-20]

About this product

Interferon regulatory factor 9 is a protein that in humans is encoded by the IRF9 gene, previously known as ISGF3G. Transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Specifications

Catalog number

HA722963

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

IFN alpha responsive transcription factor subunit antibody IFN-alpha-responsive transcription factor subunit antibody Interferon regulatory factor 9 antibody interferon stimulated transcription factor 3 antibody Interferon-stimulated gene factor 3 gamma antibody interferon-stimulated transcription factor 3, gamma 48kDa antibody IRF 9 antibody IRF-9 antibody Irf9 antibody IRF9_HUMAN antibody ISGF 3 gamma antibody ISGF-3 gamma antibody ISGF3 antibody ISGF3 p48 subunit antibody ISGF3G antibody OTTHUMP00000164692 antibody OTTHUMP00000164693 antibody p48 antibody Transcriptional regulator ISGF3 subunit gamma antibody

Abbreviation

IRF9, ISGF3G

Swiss Prot

Q00978 Human

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within human IRF9 aa 1-393.

Isotype

IgG

Clone

PSH08-20

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IP, ChIP

Positive Control

HeLa cell lysate, HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate, Daudi cell lysate, Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate, Jurkat cell lysate, Jurkat treated with 100nM IFN-α1 for 24 hours cell lysate, THP-1 cell lysate, HeLa cells treated with 10ng/mL IFN-α1 for 16 hours.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IF-Cell: 1:100 ;IP: 1-2μg/sample ;ChIP: Use 2~5 μg for 25 μg of chromatin.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 44 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes