Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit

Specifications

Catalog number

E-BC-K790-M-01

Size / count

48 T (32 samples)

Dry ice

No

Hazardous

No

Discontinued

No

Target

GAPDH

Type

Colorimetric method

Field of Research

Glycolysis and Lipid Metabolism

Assay Principle

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), a key enzyme in the glycolytic pathway, catalyzes the oxidation of glyceraldehyde-3-phosphate to 1, 3-diphosphoglycerate. This response is closely related to the gluconeogenic pathway, the maintenance of blood glucose concentrations in the body, and the development of diabetes. GAPDH gene is a housekeeping gene, which is expressed at a high level in almost all tissues. Therefore, GAPDH widely exists in tissues and cells and plays an important role in the disorders of glucose, lipid and protein metabolism in the body. This kit can calculate the enzyme activity of GAPDH by measuring the OD value change at 450 nm per unit time of the product produced by GAPDH catalyzing the oxidation of glyceraldehyde-3-phosphate.

Assay Performance Time

50 min

Sample Type

Tissue; cell

Detection Range

0.49-50.00 U/L

Sensitivity

0.49 U/L

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at -20°C for 12 months with shading light.

Inter CV

3.2-8.6%

Intra CV

3.2-5%

Detection Instrument

Microplate reader