SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified)

Catalog number S1D-C52Hf-100ug

Discontinued

Price for S1D-C52Hf-100ug

Size / count 100 µg

This variant is discontinued and cannot be added to your cart.

About this product

SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (S1D-C52Hf) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1 (T95I, G142D, E154K)). The mutations (T95I, G142D, E154K) were identified in the SARS-CoV-2 Kappa variant (Pango lineage: B.1.617.1; other names: 21A/S:154K).

Specifications

Catalog number: S1D-C52Hf-100ug
Size / count: 100 µg
Dry ice: No
Hazardous: No
Discontinued: Yes
Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 35.0 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression Region: Ser 13 - Leu 303
Host: HEK 293
Target: Spike NTD
Conjugation: Unconjugated
Tag: C-10×His
Source: SARS-CoV-2
Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin: 1.0 EU per μg
Purity: 95%
Bioactivity: Immobilized SARS-CoV-2 Spike NTD (T95I, G142D, E154K), His Tag (Cat. No. S1D-C52Hf) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M121) with a linear range of 0.1-1 ng/mL (QC tested).
Format: Powder
Buffer: PBS, pH7.4
Additives: Trehalose
Molecular Weight: 35.0 kDa
Shipping Conditions: Room Temperature
Storage Conditions: -20°C

Frequently Asked Questions

What is the catalog number for SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified)?

The catalog number for SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified) is S1D-C52Hf-100ug.

Is SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified) in stock?

SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified)?

SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified) is manufactured by CRB.

What is the estimated delivery time for SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified)?

Estimated delivery time for SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified) is approximately 10 business day(s), depending on your location.

What is SARS-CoV-2 Spike NTD Protein (T95I, G142D, E154K), His Tag (MALS verified) used for?

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.