Anti-alpha 1 Fetoprotein/AFP Antibody Picoband®

About this product

Boster Bio Anti-alpha 1 Fetoprotein/AFP Antibody Picoband® catalog # PB9090. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9090

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

AFP, also called Alpha-fetoprotein; alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.

Synonyms

Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; AFP; HPAFP

Gene Name

AFP

Gene ID

174

UniProt

P02771

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human AFP recombinant protein (Position: Q378-V609) . Human AFP shares 71% and 73% amino acid (aa) sequences identity with mouse and rat AFP, respectively.

Clonality

Polyclonal

Tissue Specificity

Plasma. Synthesized by the fetal liver and yolk sac.

Applications

IHC, WB

Field of Research

Cancer, Cardiovascular, Developmental Biology, Organogenesis, Serum Proteins, Tumor Antigens, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

References & Citations

1. Carter CS (2002) . Neuroendocrinology of sexual behavior in the female. In Becker JB. Behavioral Endocrinology. Cambridge, Mass: MIT Press. pp.88-89. ISBN 0-262-52321-3 2. Bader D, Riskin A, Vafsi O, Tamir A, Peskin B, Israel N, Merksamer R, Dar H, David M (November 2004) . Alpha-fetoprotein in the early neonatal period--a large study and review of the literature. Clin. Chim. Acta 349 (1-2) : 15–23. 3. Blair JI, Carachi R, Gupta R, Sim FG, McAllister EJ, Weston R (April 1987) . Plasma alpha fetoprotein reference ranges in infancy: effect of prematurity. Arch. Dis. Child. 62 (4) : 362–9.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68678 MW

Observed Molecular Weight

69 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Alpha-fetoprotein

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the ALB/AFP/VDB family.

Protein Name

Alpha-fetoprotein

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.