Anti-Cyclin B1/CCNB1 Antibody Picoband®

About this product

Boster Bio Anti-Cyclin B1/CCNB1 Antibody Picoband® catalog # PB9104. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9104

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CCNB also known as Cyclin B1, is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated.

Synonyms

G2/mitotic-specific cyclin-B1; CCNB1; CCNB

Gene Name

CCNB

Gene ID

891

UniProt

P14635

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433) . Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.

Clonality

Polyclonal

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Protein Phosphorylation, Signal Transduction, Tyrosine Kinases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition. .

References & Citations

1. Sartor H, Ehlert F, Grzeschik KH, Muller R, Adolph S (Aug 1992) . Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively. Genomics 13 (3) : 911–2. 2. Kimura K, Hirano M, Kobayashi R, Hirano T (October 1998) . Phosphorylation and activation of 13S condensin by Cdc2 in vitro. Science 282 (5388) : 487–90. 3. Heald R, McKeon F (May 1990) . Mutations of phosphorylation sites in lamin A that prevent nuclear lamina disassembly in mitosis. Cell 61 (4) : 579–89.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48337 MW

Observed Molecular Weight

56 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

G2/mitotic-specific cyclin-B1

Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Sequence Similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Protein Name

G2/mitotic-specific cyclin-B1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.