Anti-P Glycoprotein/ABCB1 Antibody

About this product

Boster Bio Anti-P Glycoprotein/ABCB1 Antibody catalog # RP1034. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

RP1034

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

P-GP, also called ABCB1 or PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood–brain barrier.

Synonyms

Multidrug resistance protein 1;3.6.3.44; ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD243; ABCB1; MDR1, PGY1

Gene Name

ABCB1

Gene ID

5243

UniProt

P08183

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human P Glycoprotein, different from the related rat sequence by twelve amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in liver, kidney, small intestine and brain.

Applications

IHC

Field of Research

Apoptosis, Cell Biology, Intracellular, p53 Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

References & Citations

1. Aller SG, Yu J, Ward A, Weng Y, Chittaboina S, Zhuo R, Harrell PM, Trinh YT, Zhang Q, Urbatsch IL, Chang G (March 2009) . 2. Ueda K, Clark DP, Chen CJ, Roninson IB, Gottesman MM, Pastan I (January 1987) . The human multidrug resistance (mdr1) gene. cDNA cloning and transcription initiation. J. Biol. Chem.262 (2) : 505–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

141479 MW

Observed Molecular Weight

39 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Multidrug resistance protein 1

Subcellular Location

Cell membrane ; Multi-pass membrane protein .

Sequence Similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Protein Name

Multidrug resistance protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.