- Catalog number
- PB9136
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cell adhesion molecule 1 is a protein that, in humans, is encoded by the CADM1 gene. This gene is also known as IGSF4, SYNCAM or TSLC1, and it is mapped to 11q23.3. CADM1 functions as a homophilic cell adhesion molecule at the synapse. Expression of the isolated cytoplasmic tail of CADM1 in neurons inhibited synapse assembly. Conversely, expression of full-length CADM1 in nonneuronal cells induced synapse formation by cocultured hippocampal neurons with normal release properties.
- Synonyms
- Cell adhesion molecule 1; Immunoglobulin superfamily member 4; IgSF4; Nectin-like protein 2; NECL-2; Spermatogenic immunoglobulin superfamily; SgIgSF; Synaptic cell adhesion molecule; SynCAM; Tumor suppressor in lung cancer 1; TSLC-1; CADM1 ; IGSF4 , IGSF4A, NECL2 , SYNCAM , TSLC1
- Gene Name
- CADM1
- Gene ID
- 23705
- UniProt
- Q9BY67
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- E.coli-derived human SynCAM recombinant protein (Position: Q45-P340) . Human SynCAM shares 99% amino acid (aa) sequence identity with mouse SynCAM.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level) . Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics. .
- Applications
- WB
- Field of Research
- Angiogenesis, Cardiovascular, Vasculature
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Mediates homophilic cell-cell adhesion in a Ca (2+) - independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca (2+) -independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (By similarity) . May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. .
- References & Citations
- 1. Biederer, T., Sara, Y., Mozhayeva, M., Atasoy, D., Liu, X., Kavalali, E. T., Sudhof, T. C.SynCAM, a synaptic adhesion molecule that drives synapse assembly. Science 297: 1525-1531, 2002. 2. Gomyo H, Arai Y, Tanigami A, Murakami Y, Hattori M, Hosoda F, Arai K, Aikawa Y, Tsuda H, Hirohashi S, Asakawa S, Shimizu N, Soeda E, Sakaki Y, Ohki M (Feb 2000) . A 2-Mb sequence-ready contig map and a novel immunoglobulin superfamily gene IGSF4 in the LOH region of chromosome 11q23.2. Genomics 62 (2) : 139–46. 3. Yageta, Mika; Kuramochi Masami, Masuda Mari, Fukami Takeshi, Fukuhara Hiroshi, Maruyama Tomoko, Shibuya Masabumi, Murakami Yoshinori (Sep 2002) . association of TSLC1 and DAL-1, two distinct tumor suppressor proteins in lung cancer. Cancer Res. (United States) 62 (18) : 5129–33.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 49 kDa
- Observed Molecular Weight
- 49 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Cell adhesion molecule 1
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein . Cell junction, synapse . Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder. .
- Sequence Similarities
- Belongs to the nectin family.
- Protein Name
- Cell adhesion molecule 1
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
