Anti-NFkB/NFKB2 p100/p52 Antibody Picoband®

About this product

Boster Bio Anti-NFkB/NFKB2 p100/p52 Antibody Picoband® catalog # PB9150. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9150

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NFKB2, also known as nuclear factor NF-kappa-B p100 subunit, is a protein that in humans is encoded by the NFKB2 gene. It is mapped to 10q24.32. This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFKB) . NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner.

Synonyms

Myeloid differentiation primary response protein MyD88; MYD88

Gene Name

NFKB2

Gene ID

4791

UniProt

Q00653

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human NFkB p100/p52 recombinant protein (Position: M1-R340) . Human NFkB p100/p52 shares 96% amino acid (aa) sequence identity with mouse NFkB p100/p52.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

IHC, WB

Field of Research

Adapters, Atherosclerosis, Cardiovascular, Cytoplasmic, Immunology, Innate Immunity, Signal Transduction, TLR Signaling, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. .

References & Citations

1. Schmid RM, Perkins ND, Duckett CS, Andrews PC, Nabel GJ (Sep 1991) . Cloning of an NF-kappa B subunit which stimulates HIV transcription in synergy with p65. Nature 352 (6337) : 733–6. 2. Basak, S., Kim, H., Kearns, J. D., Tergaonkar, V., O'Dea, E., Werner, S. L., Benedict, C. A., Ware, C. F., Ghosh, G., Verma, I. M., Hoffmann, A. A fourth I-kappa-B protein within the NF-kappa-B signaling module. Cell 128: 369-381, 2007. 3. Entrez Gene: NFKB2 nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

96749 MW

Observed Molecular Weight

52 kDa (active form), 120 kDa (precursor)

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)

Subcellular Location

Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) .

Sequence Similarities

Contains 7 ANK repeats.

Protein Name

Nuclear factor NF-kappa-B p100 subunit

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.