Anti-TdT/DNTT Antibody Picoband®

About this product

Boster Bio Anti-TdT/DNTT Antibody Picoband® catalog # PB9162. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9162

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template ion catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H) -D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

Synonyms

DNA nucleotidylexotransferase;2.7.7.31 ; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase ; DNTT; TDT

Gene Name

DNTT

Gene ID

1791

UniProt

P04053

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human TdT recombinant protein (Position: K316-A509) . Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

WB

Field of Research

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. .

References & Citations

1. Landau, N. R.; St. John, T. P.; Weissman, I. L.; Wolf, S. C.; Silverstone, A. E.; Baltimore, D. : Cloning of terminal transferase cDNA by antibody screening. Proc. Nat. Acad. Sci. 81: 5836-5840, 1984. 2. Yang-Feng, T. L.; Landau, N. R.; Baltimore, D.; Francke, U.: The terminal deoxynucleotidyltransferase gene is located on human chromosome 10 (10q23-q24) and on mouse chromosome 19. Cytogenet. Cell Genet. 43: 121-126, 1986. 3. Riley, L. K.; Morrow, J. K.; Danton, M. J.; Coleman, M. S.: Human terminal deoxyribonucleotidyltransferase: molecular cloning and structural analysis of the gene and 5-prime flanking region. Proc. Nat. Acad. Sci. 85: 2489-2493, 1988.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

58536 MW

Observed Molecular Weight

58 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

DNA nucleotidylexotransferase

Subcellular Location

Nucleus .

Sequence Similarities

Belongs to the DNA polymerase type-X family.

Protein Name

DNA nucleotidylexotransferase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.