Anti-Bub1 AAMP Antibody Picoband®

About this product

Boster Bio Anti-Bub1 AAMP Antibody Picoband® catalog # PB9135. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9135

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

Synonyms

Angio-associated migratory cell protein; AAMP

Gene Name

BUB1

Gene ID

699

UniProt

Q13685

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Bub1 recombinant protein (Position: V731-K1085) . Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.

Clonality

Polyclonal

Tissue Specificity

Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level) . Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics. .

Applications

WB

Field of Research

Angiogenesis, Cardiovascular, Vasculature

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a role in angiogenesis and cell migration. In smooth muscle cell migration, may act through the RhoA pathway. .

References & Citations

1. Entrez Gene: BUB1 BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) 2. Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD, Kinzler KW, Vogelstein B (March 1998) . Mutations of mitotic checkpoint genes in human cancers. Nature 392 (6673) : 300–3. 3. Roberts BT, Farr KA, Hoyt MA (December 1994) . The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol. Cell. Biol. 14 (12) : 8282–91.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46751 MW

Observed Molecular Weight

130 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Angio-associated migratory cell protein

Subcellular Location

Cell membrane. Cytoplasm.

Sequence Similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.

Protein Name

Mitotic checkpoint serine/threonine-protein kinase BUB1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.