Anti-alpha 1 Catenin/CTNNA1 Antibody Picoband®

About this product

Boster Bio Anti-alpha 1 Catenin/CTNNA1 Antibody Picoband® catalog # PB9137. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9137

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CTNNA1, also known as Catenin alpha-1 or Catenin (cadherin-associated protein), alpha 1, is a protein that in humans is encoded by the CTNNA1 gene. It is mapped to 5q31.2. When surface epithelium CTNNA1 was ablated, hair follicle development was blocked and epidermal morphogenesis was dramatically affected, with defects in adherens junction formation, intercellular adhesion, and epithelial polarity. In vitro, CTNNA1 null keratinocytes were poorly contact inhibited and grew rapidly. These differences were not dependent upon intercellular adhesion and were in marked contrast to keratinocytes conditionally null for another essential intercellular adhesion protein, desmoplakin Knockout keratinocytes exhibited sustained activation of the Ras-MAPK cascade due to aberrations in growth factor responses. It is concluded that features of precancerous lesions often attributed to defects in cell cycle regulatory genes can be generated by compromising the function of CTNNA1.

Synonyms

Catenin alpha-1; Alpha E-catenin; Cadherin-associated protein; Renal carcinoma antigen NY-REN-13; CTNNA1

Gene Name

CTNNA1

Gene ID

1495

UniProt

P35221

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292) . Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.

Clonality

Polyclonal

Tissue Specificity

Expressed ubiquitously in normal tissues.

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Actin, etc., Cadherins, Cancer, Cell Adhesion, Cytoplasmic, Cytoskeleton, Cytoskeleton/ECM, Invasion/Microenvironment, Microfilaments, Signal Transduction, Signaling Pathways, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

References & Citations

1. Vasioukhin, V., Bauer, C., Degenstein, L., Wise, B., Fuchs, E. Hyperproliferation and defects in epithelial polarity upon conditional ablation of alpha-catenin in skin. Cell 104: 605-617, 2001. 2. Herrenknecht K, Ozawa M, Eckerskorn C, Lottspeich F, Lenter M, Kemler R (November 1991) . The uvomorulin-anchorage protein alpha catenin is a vinculin homologue. Proc Natl Acad Sci U S A 88 (20) : 9156–60. 3. Entrez Gene: CTNNA1 catenin (cadherin-associated protein), alpha 1, 102kDa

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

100071 MW

Observed Molecular Weight

100 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Catenin alpha-1

Subcellular Location

Isoform 1: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.

Sequence Similarities

Belongs to the vinculin/alpha-catenin family.

Protein Name

Catenin alpha-1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.