Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband®

About this product

Boster Bio Anti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband® catalog # PB9142. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9142

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an α-chain, a β-chain, and a γ-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.

Synonyms

Laminin subunit gamma-1; Laminin B2 chain; Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; S-laminin subunit gamma; S-LAM gamma; Lamc1; Lamb-2, Lamc-1

Gene Name

LAMC1&LAMC2&LAMC3

Gene ID

3915&3918&10319

UniProt

P02468

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Peptide mixture of laminin gamma1,2,3 (DLNKLNEIEGTLNKAKDEMK; MSELEERARQQRGHLHLLET; LQRKLSLLEQESQQQELQIQ) . Laminin gamma has only three subtypes of antibody to gamma1-3 reactive with all isoforms of laminin.

Clonality

Polyclonal

Tissue Specificity

Found in the basement membranes (major component) .

Applications

IHC, IHC-F, WB

Field of Research

Angiogenesis, Cardiovascular, Cytoskeleton/ECM, Extracellular Matrix, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

References & Citations

1. Aumailley M et al. (2005) . A simplified laminin nomenclature. Matrix Biol. 24 (5) : 326–32. 2. Colognato H, Yurchenco P (2000) . Form and function: the laminin family of heterotrimers.Dev. Dyn. 218 (2) : 213–34. 3. Timpl R et al. (1979) . Laminin – a glycoprotein from basement membranes. J Biol Chem 254 (19) : 9933–7.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

177298 MW

Observed Molecular Weight

177 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Integrin beta-2

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Sequence Similarities

Belongs to the integrin beta chain family.

Protein Name

Integrin beta-2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.