Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband®

About this product

Boster Bio Anti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband® catalog # PB9151. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9151

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. It is mapped to 1p36.11. NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. NHE1 is a plausible candidate gene for human essential hypertension.

Synonyms

Sodium/hydrogen exchanger 1; APNH; Na (+) /H (+) antiporter, amiloride-sensitive; Na (+) /H (+) exchanger 1; NHE-1; Solute carrier family 9 member 1; SLC9A1; APNH1, NHE1

Gene Name

SLC9A1

Gene ID

6548

UniProt

P19634

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SLC9A1 recombinant protein (Position: H543-Q815) . Human SLC9A1 shares 92% and 93% amino acid (aa) sequences identity with mouse and rat SLC9A1, respectively.

Clonality

Polyclonal

Tissue Specificity

Kidney and intestine.

Applications

IHC, WB

Field of Research

Adapters, Atherosclerosis, Cancer, Cardiovascular, Channels, Heart Disease, Metabolism, Plasma Membrane, Signal Transduction, Transmembrane

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. .

References & Citations

1. Fliegel L, Dyck JR, Wang H, Fong C, Haworth RS (August 1993) . Cloning and analysis of the human myocardial Na+/H+ exchanger. Mol. Cell. Biochem. 125 (2) : 137–43. 2. Mattei MG, Sardet C, Franchi A, Pouysségur J (1988) . The human amiloride-sensitive Na+/H+ antiporter: localization to chromosome 1 by in situ hybridization. Cytogenet. Cell Genet. 48 (1) : 6–8. 3. Denker, S. P., Huang, D. C., Orlowski, J., Furthmayr, H., Barber, D. L. binding of the Na-H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H (+) translocation. Molec. Cell 6: 1425-1436, 2000. 4. Dudley, C. R. K., Giuffra, L. A., Tippett, P., Kidd, K. K., Reeders, S. T. The Na+/H+ antiporter: a 'melt' polymorphism allows regional mapping to the short arm of chromosome 1. Hum. Genet. 86: 79-83,1990.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

90763 MW

Observed Molecular Weight

91 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Sodium/hydrogen exchanger 1

Subcellular Location

Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane; Multi-pass membrane protein. Colocalizes with CHP1 at the reticulum endoplasmic (By similarity) . Colocalizes with CHP1 and CHP2 at the plasma membrane. .

Sequence Similarities

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

Protein Name

Sodium/hydrogen exchanger 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.