Anti-Cystathionine beta-synthase CBS Antibody Picoband®

About this product

Boster Bio Anti-Cystathionine beta-synthase CBS Antibody catalog # RP1041. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1041

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cystathionine-β-synthase, also known as CBS, is an enzyme that in humans is encoded by the CBS gene. It is mapped to 21q22.3 and contains 23 exons, ranging in size from 42 to 299 bp. CBS catalyzes the first step of the transsulfuration pathway, from homocysteine to cystathionine. It uses the cofactor pyridoxal-phosphate (PLP) and can be allosterically regulated by effectors such as the ubiquitous cofactor S-adenosyl-L-methionine (adoMet) . This enzyme belongs to the family of lyases, to be specific, the hydro-lyases, which cleave carbon-oxygen bonds. CBS is a multidomain enzyme composed of an N-terminal enzymatic domain and two CBS domains. The CBS gene is the most common locus for mutations associated with homocystinuria.

Synonyms

Cystathionine beta-synthase;4.2.1.22; Beta-thionase; Serine sulfhydrase; CBS

Gene Name

CBS

Gene ID

875

UniProt

P35520

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CBS recombinant protein (Position: A331-K551) . Human CBS shares 83% amino acid (aa) sequence identity with both mouse and rat CBS.

Clonality

Polyclonal

Tissue Specificity

In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.

Applications

IHC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, Cancer Metabolism, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury (By similarity) . .

References & Citations

1. Munke, M., Kraus, J., Watkins, P., Tanzi, R., Gusella, J., Millington Ward, A., Watson, M., Francke, U.Homocys 2. tinuria gene on human chromosome 21 mapped with cloned cystathionine beta-synthase probe and in situ hybridization of other chromosome 21 probes. (Abstract) Cytogenet. Cell Genet. 40: 706-707, 1985. 3. Kraus, J. P., Oliveriusova, J., Sokolova, J., Kraus, E., Vlcek, C., de Franchis, R., Maclean, K. N., Bao, L., Bukovska, G., Patterson, D., Paces, V., Ansorge, W., Kozich, V.The human cystathionine beta-synthase (CBS) gene: complete sequence, alternative splicing, and polymorphisms.Genomics 52: 312-324, 1998. 4. Kruger, W. D., Cox, D. R.A yeast system for expression of human cystathionine beta-synthase: structural and functional conservation of the human and yeast genes.Proc. Nat. Acad. Sci. 91: 6614-6618, 1994.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

60587 MW

Observed Molecular Weight

65 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cystathionine beta-synthase

Subcellular Location

Cytoplasm . Nucleus .

Sequence Similarities

Belongs to the cysteine synthase/cystathionine beta- synthase family.

Protein Name

Cystathionine beta-synthase

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.