Anti-CD82 Antibody Picoband®

About this product

Boster Bio Anti-CD82 Antibody Picoband® catalog # PB9170. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9170

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD82 (Cluster of Differentiation 82), also named KAI1, is a protein that in humans encoded by the CD82 gene. The gene is mapped to 11p11.2. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. The expression of CD82 protein appears to be correlated with lymph node metastasis in esophageal squamous cell carcinoma (ESCC) . And the CD82 overexpression can suppress tumor invasiveness and metastatic potential by inducing MMP9 inactivation via upregulation of TIMP1.

Synonyms

CD82 antigen; C33 antigen; IA4; Inducible membrane protein R2; Metastasis suppressor Kangai-1; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; Tspan-27; CD82; CD82; KAI1, SAR2, ST6, TSPAN27

Gene Name

CD82

Gene ID

3732

UniProt

P27701

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CD82 recombinant protein (Position: A98-Y267) . Human CD82 shares 69% and 67% amino acid (aa) sequences identity with mouse and rat CD82, respectively.

Clonality

Polyclonal

Tissue Specificity

Lymphoid specific.

Applications

IHC, WB

Field of Research

Adaptive Immunity, Cancer, Cancer Susceptibility, Cell Type Markers, Epigenetics and Nuclear Signaling, Immunology, NK Cells, Oncoproteins/Suppressors, Platelets, T Cells, Transcription, Tumor Suppressors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.

References & Citations

1. Dong, J.-T.; Lamb, P. W.; Rinker-Schaeffer, C. W.; Vukanovic, J.; Ichikawa, T.; Isaacs, J. T.; Barrett, J. C. : KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2. Science 268: 884-886, 1995. 2. Mashimo, T.; Watabe, M.; Hirota, S.; Hosobe, S.; Miura, K.; Tegtmeyer, P. J.; Rinker-Shaeffer, C. W.; Watabe, K. : The expression of the KAI1 gene, a tumor metastasis suppressor, is ly activated by p53. Proc. Nat. Acad. Sci. 95: 11307-11311, 1998. 3. Miyazaki, T.; Kato, H.; Shitara, Y.; Yoshikawa, M.; Tajima, K.; Masuda, N.; Shouji, H.; Tsukada, K.; Nakajima, T.; Kuwano, H. : Mutation and expression of the metastasis suppressor gene KAI1 in esophageal squamous cell carcinoma. Cancer 89: 955-962, 2000. 4. Jee, B. K.; Park, K. M.; Surendran, S.; Lee, W. K.; Han, C. W.; Kim, Y. S.; Lim, Y. : KAI1/CD82 suppresses tumor invasion by MMP9 inactivation via TIMP1 up-regulation in the H1299 human lung carcinoma cell line. Biochem. Biophys. Res. Commun. 342: 655-661, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29626 MW

Observed Molecular Weight

30-36 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

CD82 antigen

Subcellular Location

Membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the tetraspanin (TM4SF) family.

Protein Name

CD82 antigen

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.