Anti-Claudin 2/CLDN2 Antibody Picoband®

About this product

Boster Bio Anti-Claudin 2/CLDN2 Antibody catalog # RP1042. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1042

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.

Synonyms

Claudin-2; SP82; CLDN2; PSEC0059, SP82, UNQ705/PRO1356

Gene Name

CLDN2

Gene ID

9075

UniProt

P57739

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Claudin 2 recombinant protein (Position: A38-V230) . Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2.

Clonality

Polyclonal

Applications

IHC, WB

Field of Research

Cell Adhesion, Cytoskeleton/ECM, Signal Transduction, Tight Junctions

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. .

References & Citations

1. Morita K, Furuse M, Fujimoto K, Tsukita S (Mar 1999) . Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. Proc Natl Acad Sci U S A 96 (2) : 511–6. 2. Sakaguchi, T., Gu, X., Golden, H. M., Suh, E., Rhoads, D. B., Reinecker, H.-C. Cloning of the human claudin-2 5-prime-flanking region revealed a TATA-less promoter with conserved binding sites in mouse and human for caudal-related homeodomain proteins and hepatocyte nuclear factor-1-alpha. J. Biol. Chem. 277: 21361-21370, 2002. 3. Muto, S.; Hata, M.; Taniguchi, J.; Tsuruoka, S.; Moriwaki, K.; Saitou, M.; Furuse, K.; Sasaki, H.; Fujimura, A.; Imai, M.; Kusano, E.; Tsukita, S.; Furuse, M. (2010) . Claudin-2-deficient mice are defective in the leaky and cation-selective paracellular permeability properties of renal proximal tubules. Proceedings of the National Academy of Sciences 107 (17) : 8011–8016.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

24549 MW

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Claudin-2

Subcellular Location

Cell junction, tight junction . Cell membrane ; Multi-pass membrane protein .

Protein Name

Claudin-2

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.