Anti-Dihydrofolate reductase (DHFR) Antibody Picoband®

About this product

Boster Bio Anti-Dihydrofolate reductase (DHFR) Antibody Picoband® catalog # PB9175. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9175

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5. What's more, DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Synonyms

Dihydrofolate reductase;1.5.1.3; DHFR

Gene Name

DHFR

Gene ID

1719

UniProt

P00374

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human DHFR recombinant protein (Position: V2-D187) . Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.

Clonality

Polyclonal

Tissue Specificity

Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Amino Acid Metabolism, Amino Acids, Cancer, Cell Biology, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. .

References & Citations

1. Chen MJ, Shimada T, Moulton AD, Harrison M, Nienhuis AW (December 1982) . Intronless human dihydrofolate reductase genes are derived from processed RNA molecules. Proc. Natl. Acad. Sci. U.S.A. 79 (23) : 7435–9. 2. Chen MJ, Shimada T, Moulton AD, Cline A, Humphries RK, Maizel J, Nienhuis AW (March 1984) . The functional human dihydrofolate reductase gene. J. Biol. Chem. 259 (6) : 3933–43. 3. Funanage VL, Myoda TT, Moses PA, Cowell HR (October 1984) . Assignment of the human dihydrofolate reductase gene to the q11----q22 region of chromosome 5. Mol. Cell. Biol. 4 (10) : 2010–6.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21453 MW

Observed Molecular Weight

21 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Dihydrofolate reductase

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sequence Similarities

Belongs to the dihydrofolate reductase family.

Protein Name

Dihydrofolate reductase

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.