Anti-Dicer/DICER1 Antibody Picoband®

About this product

Boster Bio Anti-Dicer/DICER1 Antibody catalog # RP1044. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1044

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dicer (DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC) . It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

Synonyms

Endoribonuclease Dicer;3.1.26.3; Helicase with RNase motif; Helicase MOI; DICER1; DICER, HERNA, KIAA0928

Gene Name

DICER1

Gene ID

23405

UniProt

Q9UPY3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Dicer recombinant protein (Position: M1-N195) . Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer.

Clonality

Polyclonal

Applications

Flow Cytometry, IHC, WB

Field of Research

DNA/RNA, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, RNA Processing

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to the RNA- induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes. .

References & Citations

1. Hutvagner, G., McLachlan, J., Pasquinelli, A. E., Balint, E., Tuschl, T., Zamore, P. D. A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA. Science 293: 834-838, 2001. 2. Matsuda, S., Ichigotani, Y., Okuda, T., Irimura, T., Nakatsugawa, S., Hamaguchi, M. Molecular cloning and characterization of a novel human gene (HERNA) which encodes a putative RNA-helicase. Biochim. Biophys. Acta 1490: 163-169, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

218682 MW

Observed Molecular Weight

250 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Endoribonuclease Dicer

Subcellular Location

Cytoplasm .

Sequence Similarities

Belongs to the helicase family. Dicer subfamily.

Protein Name

Endoribonuclease Dicer

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.