- Catalog number
- PB9181
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Bis (5'-adenosyl) -triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1, P3-bis (5'-adenosyl) -triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.
- Synonyms
- Bis (5'-adenosyl) -triphosphatase;3.6.1.29; AP3A hydrolase; AP3Aase; Diadenosine 5',5'''-P1, P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT
- Gene Name
- FHIT
- Gene ID
- 2272
- UniProt
- P49789
- Host
- Rabbit
- Reactivity
- Human, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- E.coli-derived human FHIT recombinant protein (Position: M1-Q147) . Human FHIT shares 90% and 87% amino acid (aa) sequences identity with mouse and rat FHIT, respectively.
- Clonality
- Polyclonal
- Tissue Specificity
- Low levels expressed in all tissues tested. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines.
- Applications
- Flow Cytometry, IF, IHC, ICC, WB
- Field of Research
- Cancer Susceptibility, Epigenetics and Nuclear Signaling, Transcription, Tumor Suppressors
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Cleaves P (1) -P (3) -bis (5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P (1) -P (4) -bis (5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P (1) -P (3) -bis (5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low- affinity Ca (2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor. .
- References & Citations
- 1. Ohta M, Inoue H, Cotticelli MG, Kastury K, Baffa R, Palazzo J, Siprashvili Z, Mori M, McCue P, Druck T, Croce CM, Huebner K (Apr 1996) . The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t (3;8) breakpoint, is abnormal in digestive tract cancers. Cell 84 (4) : 587–97. 2. Pekarsky Y, Campiglio M, Siprashvili Z, Druck T, Sedkov Y, Tillib S, Draganescu A, Wermuth P, Rothman JH, Huebner K, Buchberg AM, Mazo A, Brenner C, Croce CM (Aug 1998) . Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans. Proc Natl Acad Sci U S A 95 (15) : 8744–9. 3. Pollard KS, Salama SR, Lambert N, Lambot MA, Coppens S, Pedersen JS, Katzman S, King B, Onodera C, Siepel A, Kern AD, Dehay C, Igel H, Ares M Jr, Vanderhaeghen P, Haussler D (2006-08-16) . An RNA gene expressed during cortical development evolved rapidly in humans. Nature 443 (7108) : 167–172.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 16858 MW
- Observed Molecular Weight
- 17 kDa
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- 6
- Gene Name Synonym
- Bis (5'-adenosyl) -triphosphatase
- Subcellular Location
- Cytoplasm. Mitochondrion. Nucleus.
- Sequence Similarities
- Contains 1 HIT domain.
- Protein Name
- Bis (5'-adenosyl) -triphosphatase
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
