Anti-gamma Catenin/JUP Antibody Picoband®

About this product

Boster Bio Anti-gamma Catenin/JUP Antibody Picoband® catalog # PB9185. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9185

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Junction plakoglobin (JUP), also known as gamma-catenin, is a protein that in humans is encoded by the JUP gene. It is a member of the catenin protein family and homologous to β -catenin, and it is mapped to 17q21.2. This gene encodes a major cytoplasmic protein that is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins. Meanwhile, JUP may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.

Synonyms

Junction plakoglobin; Catenin gamma; Desmoplakin III; Desmoplakin-3; JUP; CTNNG, DP3

Gene Name

JUP

Gene ID

3728

UniProt

P14923

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human gamma Catenin recombinant protein (Position: M556-A745) . Human gamma Catenin shares 98% amino acid (aa) sequence identity with both mouse and rat gamma Catenin.

Clonality

Polyclonal

Tissue Specificity

Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. .

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity) . .

References & Citations

1. Kolligs, F. T.; Kolligs, B.; Hajra, K. M.; Hu, G.; Tani, M.; Cho, K. R.; Fearon, E. R. : Gamma-catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta-catenin. Genes Dev. 14: 1319-1331, 2000. 2. Entrez Gene: JUP junction plakoglobin

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

81745 MW

Observed Molecular Weight

82 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Junction plakoglobin

Subcellular Location

Cell junction, adherens junction . Cell junction, desmosome . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Cytoplasmic in a soluble and membrane-associated form.

Sequence Similarities

Belongs to the beta-catenin family.

Protein Name

Junction plakoglobin

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.