Anti-p73/TP73 Antibody Picoband®

About this product

Boster Bio Anti-p73/TP73 Antibody Picoband® catalog # PB9235. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9235

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

P73, also known as tumor protein 73 (TP73), encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It is mapped to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Furthermore, recent finding are suggesting that over-expression of transcription factors involved in cell cycle regulation and synthesis of DNA in mammalian cells (e.g.: E2F-1) induces the expression of p73. In addition, p73 is a substrate of the c-Abl kinase and that the ability of c-Abl to phosphorylate p73 is markedly increased by gamma-irradiation.

Synonyms

Tumor protein p73; p53-like transcription factor; p53-related protein; TP73; P73

Gene Name

TP73

Gene ID

7161

UniProt

O15350

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human p73 recombinant protein (Position: M1-E198) . Human p73 shares 96% amino acid (aa) sequence identity with mouse p73.

Clonality

Polyclonal

Tissue Specificity

Expressed in striatal neurons of patients with Huntington disease (at protein level) . Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. .

Applications

IHC, WB

Field of Research

Apoptosis, Cell Biology, Intracellular, p53 Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. .

References & Citations

1. Kaghad, M.; Bonnet, H.; Yang, A.; Creancier, L.; Biscan, J.-C.; Valent, A.; Minty, A.; Chalon, P.; Lelias, J.-M.; Dumont, X.; Ferrara, P.; McKeon, F.; Caput, D. : Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers. Cell 90: 809-819, 1997. 2. Agami, R.; Blandino, G.; Oren, M.; Shaul, Y. : Interaction of c-Abl and p73-alpha and their collaboration to induce apoptosis. Nature 399: 809-813, 1999. 3. Yuan, Z.-M.; Shioya, H.; Ishiko, T.; Sun, X.; Gu, J.; Huang, Y. Y.; Lu, H.; Kharbanda, S.; Weichselbaum, R.; Kufe, D. : p73 is regulated by tyrosine kinase c-Abl in the apoptotic response to DNA damage. Nature 399: 814-817, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

69623 MW

Observed Molecular Weight

73 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Tumor protein p73

Subcellular Location

Nucleus . Cytoplasm. Accumulates in the nucleus in response to DNA damage.

Sequence Similarities

Belongs to the p53 family.

Protein Name

Tumor protein p73

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.