Anti-Transferrin Receptor/TFRC Antibody Picoband®

About this product

Boster Bio Anti-Transferrin Receptor/TFRC Antibody Picoband® catalog # PB9233. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9233

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transferrin receptor protein 1 (TfR1), also known as Cluster of Differentiation 71 (CD71), is a protein that in humans is encoded by the TFRC gene. It is mapped to 3q29. TFRC is a transmembrane glycoprotein composed of two disulfide-linked monomers joined by two disulfide bonds. Expression of human TFR1 in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the glycoprotein of Machupo, Guanarito, and Junin viruses. TFR1 is a cellular receptor for New World hemorrhagic fever arenaviruses. It is required for iron delivery from transferrin to cells.

Synonyms

Transferrin receptor protein 1; TR; TfR; TfR1; Trfr; T9; p90; CD71; Transferrin receptor protein 1, serum form; sTfR; TFRC

Gene Name

TFRC

Gene ID

7037

UniProt

P02786

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human TFRC recombinant protein (Position: M1-N198) . Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. .

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Serum Proteins, Stem Cells, Surface Molecules, T Lymphocytic Lineage

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity) . A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. .

References & Citations

1. Sutherland R, Delia D, Schneider C, Newman R, Kemshead J, Greaves M (July 1981) . Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin 2. Speeckaert MM, Speeckaert R, Delanghe JR (December 2010) . Biological and clinical aspects of soluble transferrin receptor. Critical Reviews in Clinical Laboratory Sciences 47 (5-6) : 213–228. 3. Radoshitzky, S. R., Abraham, J., Spiropoulou, C. F., Kuhn, J. H., Nguyen, D., Li, W., Nagel, J., Schmidt, P. J., Nunberg, J. H., Andrews, N. C., Farzan, M., Choe, H. Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses. Nature 446: 92-96, 2007.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

84871 MW

Observed Molecular Weight

95 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Transferrin receptor protein 1

Subcellular Location

Cell membrane ; Single-pass type II membrane protein . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Sequence Similarities

Belongs to the peptidase M28 family. M28B subfamily.

Protein Name

Transferrin receptor protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.