Anti-KLF6 Antibody Picoband®

About this product

Boster Bio Anti-KLF6 Antibody Picoband® catalog # PB9231. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9231

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Krueppel-like factor 6 (KLF6) is a protein that in humans is encoded by the KLF6 gene. It is a tumor suppressor gene which is located on 10p15.1. This gene encodes a nuclear protein that has three zinc fingers at the end of its C-terminal domain, a serine/threonine-rich central region, and an acidic domain lying within the N-terminal region. The zinc fingers of this protein are responsible for the specific DNA binding with the guanine-rich core promoter elements. The central region might be involved in activation or posttranslational regulatory pathways, and the acidic N-terminal domain might play an important role in the process of transcriptional activation. It is capable of activating transcription approximately 4-fold either on homologous or heterologous promoters. The DNA binding and transcriptional activity of this protein, in conjunction with its expression pattern, suggests that this protein may participate in the regulation and/or maintenance of the basal expression of pregnancy-specific glycoprotein genes and possibly other TATA box-less genes.

Synonyms

Krueppel-like factor 6; B-cell-derived protein 1; Core promoter element-binding protein; GC-rich sites-binding factor GBF; Proto-oncogene BCD1; Suppressor of tumorigenicity 12 protein; Transcription factor Zf9; KLF6; BCD1, COPEB, CPBP, ST12

Gene Name

KLF6

Gene ID

1316

UniProt

Q99612

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human KLF6 recombinant protein (Position: E38-N205) . Human KLF6 shares 92% and 90% amino acid (aa) sequences identity with mouse and rat KLF6, respectively.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. Weakly expressed in pancreas, lung, liver, heart and skeletal muscle. Also expressed in fetal brain, spleen and thymus.

Applications

WB

Field of Research

Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Hematopoietic Progenitors, Krueppel Like Factor, Stem Cells, Transcription, Zinc Finger

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional activator (By similarity) . Binds a GC box motif. Could play a role in B-cell growth and development. .

References & Citations

1. Onyango P, Koritschoner NP, Patrito LC, Zenke M, Weith A (Apr 1998) . Assignment of the gene encoding the core promoter element binding protein (COPEB) to human chromosome 10p15 by somatic hybrid analysis and fluorescence in situ hybridization. Genomics 48 (1) : 143–4. 2. Li D; Yea S; Dolios G et al. (October 2005) . Regulation of Kruppel-like factor 6 tumor suppressor activity by acetylation. Cancer Res. 65 (20) : 9216–25. 3. Entrez Gene: KLF6 Kruppel-like factor 6

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

31865 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Krueppel-like factor 6

Subcellular Location

Nucleus.

Sequence Similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein Name

Krueppel-like factor 6

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.