Anti-MCAK/KIF2C Antibody Picoband®

About this product

Boster Bio Anti-MCAK/KIF2C Antibody Picoband® catalog # PB9230. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9230

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kinesin-like protein KIF2C is a protein that in humans is encoded by the KIF2C gene. It is mapped to 1p34.1. The protein encoded by this gene is a member of kinesin-like protein family. Most proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. KIF2C uses microtubule depolymerizing activity to correct improper microtubule attachments at kinetochores.

Synonyms

Kinesin-like protein KIF2C; Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK; KIF2C; KNSL6

Gene Name

KIF2C

Gene ID

11004

UniProt

Q99661

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human MCAK recombinant protein (Position: G531-Q725) . Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK.

Clonality

Polyclonal

Tissue Specificity

Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. .

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cytoskeleton, Cytoskeleton/ECM, Motor Proteins, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. .

References & Citations

1. Manning, A. L., Ganem, N. J., Bakhoum, S. F., Wagenbach, M., Wordeman, L., Compton, D. A. The kinesin-13 proteins, Kif2a, Kif2b, and Kif2c/MCAK have distinct roles during mitosis in human cells. Molec. Biol. Cell 18: 2970-2979, 2007. 2. Kim IG, Jun DY, Sohn U, Kim YH (Feb 1998) . Cloning and expression of human mitotic centromere-associated kinesin gene. Biochim Biophys Acta 1359 (3) : 181–6. 3. Entrez Gene: KIF2C kinesin family member 2C

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

81313 MW

Observed Molecular Weight

81 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Kinesin-like protein KIF2C

Subcellular Location

Cytoplasm, cytoskeleton . Nucleus . Chromosome, centromere. Chromosome, centromere, kinetochore. Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity) . Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGOL2. .

Sequence Similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.

Protein Name

Kinesin-like protein KIF2C

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.