Anti-FOXO3A Antibody Picoband®

About this product

Boster Bio Anti-FOXO3A Antibody Picoband® catalog # PB9196. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9196

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Forkhead box O3, also known as FKHRL1 or FOXO3a, is a human protein encoded by the FOXO3 gene. FOXO3 belongs to the O subclass of the forkhead family of transcription factors which are characterized by a distinct fork head DNA-binding domain. It is mapped to 6q21. This protein likely functions as a trigger for apoptosis through upregulation of genes necessary for cell death, such as Bim and PUMA, or downregulation of anti-apoptotic proteins such as FLIP. In mammals FOXO3 regulates the resistance of cells to stress by inducing DNA repair and thereby may also affect organismal life span. In addition, it is thought that FOXO3 is also involved in protection from oxidative stress by upregulating antioxidants such as catalase and MnSOD.

Synonyms

Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1, FOXO3A

Gene Name

FOXO3

Gene ID

2309

UniProt

O43524

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human FOXO3A recombinant protein (Position: Q471-G673) . Human FOXO3A shares 97% amino acid (aa) sequence identity with mouse FOXO3A.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. .

Applications

IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Co-Activators/Co-Repressors, Domain Families, Epigenetics and Nuclear Signaling, Forkhead Box, Hypoxia, Intracellular, Metabolism, Metabolism Processes, Nuclear Receptors, Nuclear Signaling Pathways, Oncoproteins, Oncoproteins/Suppressors, Pathways and Processes, Transcription, Transcription Factors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. .

References & Citations

1. Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC (January 1998) . Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics 47 (2) : 187–99. 2. Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME (March 1999) . Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Cell 96 (6) : 857–68. 3. Ekoff M, Kaufmann T, Engström M, Motoyama N, Villunger A, Jönsson JI, Strasser A, Nilsson G (November 2007) . The BH3-only protein Puma plays an essential role in cytokine deprivation induced apoptosis of mast cells. Blood 110 (9) : 3209–17.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

71277 MW

Observed Molecular Weight

80-90 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Forkhead box protein O3

Subcellular Location

Cytoplasm, cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.

Sequence Similarities

Contains 1 fork-head DNA-binding domain.

Protein Name

Forkhead box protein O3

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.