Anti-GCLC Antibody Picoband®

About this product

Boster Bio Anti-GCLC Antibody Picoband® catalog # PB9201. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9201

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

GCLC, also named Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Synonyms

Glutamate--cysteine ligase catalytic subunit;6.3.2.2; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCLC; GLCL, GLCLC

Gene Name

GCLC

Gene ID

2729

UniProt

P48506

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human GCLC recombinant protein (Position: E437-N637) . Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC.

Clonality

Polyclonal

Tissue Specificity

T-cells and endothelial cells.

Applications

Flow Cytometry, IHC, WB

Field of Research

Cancer, Cell Biology, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor required during cardiovascular development (PubMed:23289003) . Plays an important role in the transcriptional program (s) that underlies smooth muscle cell diversity (By similarity) . Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer (PubMed:25543888) . .

References & Citations

1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35, 1992. 2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M. L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

72766 MW

Observed Molecular Weight

72 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Glutamate--cysteine ligase catalytic subunit

Subcellular Location

Nucleus .

Sequence Similarities

Belongs to the glutamate--cysteine ligase type 3 family.

Protein Name

Glutamate--cysteine ligase catalytic subunit

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.