Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband®

About this product

Boster Bio Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband® catalog # PB9212. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9212

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HMOX1 (heme oxygenase (decycling) 1), also known as HO-1, is a human gene that encodes for the enzyme heme oxygenase 1. It is an essential enzyme in heme catabolism, it cleaves heme to form biliverdin. HMOX1 belongs to the heme oxygenase family. The HMOX1 gene is located on the long (q) arm of chromosome 22 at position 12.3, from base pair 34,101,636 to base pair 34,114,748. HMOX1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HMOX1 activity is induced by its substrate heme and by various nonheme substances.

Synonyms

Heme oxygenase 1; HO-1;1.14.99.3; HMOX1; HO, HO1

Gene Name

HMOX1

Gene ID

3162

UniProt

P09601

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human HMOX1 recombinant protein (Position: M1-M288) . Human HMOX1 shares 82% and 80% amino acid (aa) sequences identity with mouse and rat HMOX1, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level) . .

Applications

IHC, WB

Field of Research

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Epigenetics and Nuclear Signaling, Heart Disease, Metabolism, Metabolism Processes, Neurodegenerative Disease, Neurology Process, Neuroscience, NFKB Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Pathways and Processes, Platelets, Response To Hypoxia, Signal Transduction, Signaling Pathway, Vascular Inflammation, Vasculature

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

References & Citations

1. He, C. H., Gong, P., Hu, B., Stewart, D., Choi, M. E., Choi, A. M. K., Alam, J.Identification of activating transcription factor 4 (ATF4) as an Nrf2-interacting protein: implication for heme oxygenase-1 gene regulation. J. Biol. Chem. 276: 20858-20865, 2001. 2. Piantadosi CA, Withers CM, Bartz RR, MacGarvey NC, Fu P, Sweeney TE, Welty-Wolf KE, Suliman HB (May 2011) . Heme oxygenase-1 couples activation of mitochondrial biogenesis to anti-inflammatory cytokine expression. J. Biol. Chem.286 (18) : 16374–85.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

32819 MW

Observed Molecular Weight

33 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Heme oxygenase 1

Subcellular Location

Microsome . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side .

Sequence Similarities

Belongs to the heme oxygenase family.

Protein Name

Heme oxygenase 1

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.